(617ay) Enhanced Carbon Sequestration with Cascade Enzymtic Reactions

Authors: 
Xia, S., University of Minnesota
Wang, P., University of Minnesota
Carbon capture capacity is an important factor of the feasibility of carbon capture and sequestration process. Almost complete conversion would be achieved by chemical carbon capture while most biocatalytic reactions reach much lower degree of conversion as limited by reaction equilibrium. In order to achieve high conversion rate in biocatalytic reactions, cascade enzyme reaction designed for carbon capture in a homogeneous solution can further the conversion of substrate, thus improving the overall carbon capture capacity. By integrating the reactions of two key enzymes of RTCA cycle, isocitrate dehydrogenase and aconitase, we demonstrate that intensified carbon capture can be realized through such cascade enzymatcic reactions. With cascade reaction, 0.5 mol carbon could be captured at pH 6 for each mole of substrate applied, compared with only 0.2 mol carbon with single isocitrate dehydrogenase. Overal it manifests tat carbon capture capacity of biocatalytic reactions cab be intensified with cascade reactions.