(47g) Laccase: Fermentation Process Intensification and Efficient Purification By Magnetic Separation

A promising farnesol addition strategy was developed to enhance the laccase production from Trametes versicolor, and the laccase fermentation was successfully scaled up to a 2 m³ stirred-tank reactor. The addition of farnesol resulted in an increased expression of the laccase (lac) gene and the RhoA gene. The RhoA gene correlated with hyperbranched mycelia, which facilitated the secretion of the intracellular laccase. An emphasis is highlighted on direct capture of laccase from T. versicolor fermentation broth using magnetic mesoporous silica nanoparticles with metal affinity ligands. The Cu²⁺-chelated magnetic mesoporous silica nanoparticles with an average pore size of 14.5 nm provide 60.6-fold purification of laccase and 114.6% recovery yield of enzyme activity. The simple and efficient bioseparation method has great potential to be used for the robust and inexpensive preparation of purified laccase at the industrial scale.