(256c) Development of a Microdevice-Based Human Mesenchymal Stem Cell-Mediated Drug-Delivery System

Development
of a Microdevice-based Human Mesenchymal Stem Cell-mediated Drug-Delivery
System

Junfei Xia^a, Ang-Chen Tsai^a, Teng
Ma^a, Jingjiao Guan^a,b

^a Department of Chemical and
Biomedical Engineering, FAMU-FSU College of Engineering, Florida State University

^b Integrative NanoScience Institute,
Florida State University

Abstract

Cell-mediated drug delivery allows
to harness unique abilities of living cells to achieve controlled delivery of
drugs, but traditional cell-mediated drug delivery techniques suffer drawbacks
such as risk of intracellular degradation of drug payload or low drug loading
capacity. These limitations can be potentially overcome by attaching a
disk-shaped drug-laden microparticle termed microdevice to the external surface
of a cell. On the other hand, human mesenchymal stem cell (hMSC) is a primary
candidate for next-generation cell therapies. It is therefore an ideal cell
type for cell-mediated drug delivery. In this presentation, we introduce
production and characterization of a novel microdevice-based hMSC-mediated
drug-delivery system for the first time. In particular, we present in vitro
studies on how microdevice attachment affects viability, migration, stemness,
capability of forming spheroid of the hMSCs. Successful development of this
microdevice-based hMSC-mediated drug delivery system may lead to a new
therapies for treating many human diseases.

Fig.1 Left: Schematic illustration of cell-microdevice complex. Right: single human mesenchymal stem cell in suspension attached by microdevices.
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Keywords: cell-mediated drug delivery,
human mesenchymal stem cell, drug delivery