(224c) Nanoharvesting of Polyphenolic Flavonoids from Solidago Nemoralis Hairy Root Cultures Using Functionalized Mesoporous Silica Nanoparticles
- Conference: AIChE Annual Meeting
- Year: 2016
- Proceeding: 2016 AIChE Annual Meeting
- Group: Nanomaterials for Applications in Energy and Biology
Monday, November 14, 2016 - 4:05pm-4:30pm
Here, nanoharvesting of polyphenolic flavonoids, enriched in genetically modified S. nemoralis hairy root cultures, is carried out using mesoporous silica nanoparticles (MSNPs) functionalized with both amines and titanium dioxide (TiO2). Amine functionalization was performed to facilitate uptake of nanoparticles into plant cells, and TiO2 functionalization to provide coordination binding sites for metabolites. Non-magnetic MSNPs (170 nm diameter) and magnetic MSNPs with Fe3O4 cores (70 nm diameter) with highly porous structures were synthesized and functionalized with amines and TiO2. Both particles were shown to be taken up in S. nemoralis hairy roots, and recovered using external centrifugal and magnetic forces, respectively. Intracellular uptake and localization of the nanoparticles (0-1000 µg/ml in Murashige and Skoog media) in hairy roots were visualized by fluorescent imaging, after tagging the nanoparticles with rhodamine B isothiocyanate. Quenching of fluorescence in bulk solution using trypan blue was used to confirm intracellular localization of the tagged particles. Post-uptake viability of hairy roots was demonstrated by a concentration-dependent growth study. Proof of the flavonoid nanoharvesting concept was inferred from the color change (white to orange) of the recovered nanoparticles after exposure to hairy roots. Quantitative determination and analysis of nanoharvested flavonoids will be reported based on antiradical activity of metabolites bound to particles and HPLC.