(620bu) Regulation of Carbon Metabolism and Lipid Accumulation in Yarrowia Lipolytica (Rapid Fire)

Regulation of Carbon Metabolism and
Lipid Accumulation in Yarrowia lipolytica

            Oleaginous yeasts are species that naturally
accumulate high titers of lipid molecules[1, 2].
These are of particular interest because lipids and lipid derived molecules are
attractive alternatives to petroleum based chemicals with applications ranging
from fuels to food additives[3, 4].
The natural lipid accumulation, however, is known to occur when the growth
media contains a high ratio of carbon to nitrogen. Furthermore, lipid
accumulation in Y. lipolytica occurs only after a lag phase in which
sugar is consumed but lipid is not produced. It has been shown that the deletion
of the global AMPK type regulator Snf1 results in the abolition of the lipid
accumulation lag phase as well as an increase in the amount of lipid produced[5]. We conducted studies on regulation of
substrate transporters as well as enzymes in the lipid metabolism pathway to
elucidate the mechanism by which lipid accumulation is controlled in Y.
lipolytica.

1.         Tai, M. and G. Stephanopoulos, Engineering the
push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for
biofuel production. Metabolic engineering, 2013. 15: p. 1-9.

2.         Ageitos,
J.M., J.A. Vallejo, P. Veiga-Crespo, and T.G. Villa, Oily yeasts as
oleaginous cell factories. Applied Microbiology and Biotechnology, 2011. 90(4):
p. 1219-1227.

3.         Santos,
T.M., A. Pinto, A.V. Oliveira, H.L. Ribeiro, C.A. Caceres, E.N. Ito, and H.
Azeredo, Physical properties of cassava starch?carnauba wax emulsion films
as affected by component proportions. International Journal of Food Science
& Technology, 2014.

4.         Moser,
B.R., Biodiesel production, properties, and feedstocks, in Biofuels.
2011, Springer. p. 285-347.

5.         Seip,
J., R. Jackson, H. He, Q. Zhu, and S.-P. Hong, Snf1 Is a Regulator of Lipid
Accumulation in Yarrowia lipolytica. Applied and environmental
microbiology, 2013. 79(23): p. 7360-7370.