(620ak) Reconfigurable Hybrid Interface for Molecular Marker Diagnostics and in-Situ Reporting (Rapid Fire) Conference: AIChE Annual MeetingYear: 2015Proceeding: 2015 AIChE Annual MeetingGroup: Food, Pharmaceutical & Bioengineering DivisionSession: Poster Session: Bioengineering Time: Wednesday, November 11, 2015 - 6:00pm-8:00pm Authors: Ehrhardt, K., The University of Texas at Dallas Guinn, M., The University of Texas at Dallas Quarton, T., The University of Texas at Dallas Zhang, M. Q., The University of Texas at Dallas Bleris, L., The University of Texas at Dallas Combinations of molecular signals such as transcription factors and microRNAs in cells are a reliable indicator of multi-gene disorders. A system capable of detecting these conditions in-situ may be used as a tool for diagnosis and monitoring of disease. Here, we engineer genetic circuits that sense endogenous levels of the androgen receptor (AR), the glucocorticoid receptor (GR), and the microRNA hsa-miR-21 (miR-21) in cervical cancer cells (HeLa). Furthermore, using the mediator molecule human chorionic gonadotropin (hCG), we interface the intracellular information to enzyme-linked immunosorbent assay (ELISA) test strips. We demonstrate that this hybrid genetic circuit and test-strip interface can accommodate combinatorial, low-cost, and in-situ reporting, a versatile profiling tool.