(620ak) Reconfigurable Hybrid Interface for Molecular Marker Diagnostics and in-Situ Reporting (Rapid Fire)

Authors: 
Ehrhardt, K., The University of Texas at Dallas
Guinn, M., The University of Texas at Dallas
Quarton, T., The University of Texas at Dallas
Zhang, M. Q., The University of Texas at Dallas
Bleris, L., The University of Texas at Dallas

Combinations of molecular signals such as transcription factors and microRNAs in cells are a reliable indicator of multi-gene disorders. A system capable of detecting these conditions in-situ may be used as a tool for diagnosis and monitoring of disease. Here, we engineer genetic circuits that sense endogenous levels of the androgen receptor (AR), the glucocorticoid receptor (GR), and the microRNA hsa-miR-21 (miR-21) in cervical cancer cells (HeLa). Furthermore, using the mediator molecule human chorionic gonadotropin (hCG), we interface the intracellular information to enzyme-linked immunosorbent assay (ELISA) test strips. We demonstrate that this hybrid genetic circuit and test-strip interface can accommodate combinatorial, low-cost, and in-situ reporting, a versatile profiling tool.