(193c) Using the Personal Glucose Meter to Quantify a Wide Range of Targets

Using the personal glucose meter for
convenient quantification of wide range of targets

Tian Lan, Yu
Xiang and Yi LU

Abstract:

Convenient, low-cost and quantitative detection of broad
range of targets has the great potential to revolutionize today's practice for medical
and environmental monitoring. Despite years of research, very few such devices
have been commercialized at a large scale. Today's personal glucose meter (PGM)
is the culmination of decades of R&D and commercialization efforts aimed to
satisfy the daily need of millions of diabetes patients worldwide to monitor
their blood glucose levels. The PGM is the almost the ideal sensing platform,
which offers convenience, low-cost and quantitative, except it only quantifies
one target, glucose.

Instead of designing a new platform that can
be time consuming and costly, we have developed an innovative method that
adapts the widely available PGM for quantifying a wide range of targets,
including metal ions, small molecules, proteins and nucleic acid sequences. An
enzyme invertase, which hydrolyzes sucrose into glucose, is conjugated to
recognition molecules, such as aptamers, DNAzymes, antibodies and DNA, and relays
the non-glucose target binding events to the presence and concentration of glucose,
which can be measured using PGM. Using this approach, we have demonstrated
quantification of metal ions using DNAzymes, small molecules using aptamers and
antibodies, various protein markers using antibodies, and viral DNA fragments
using complementary DNA as shown in Table-1.

In conclusion, we have demonstrated an innovative way of
leveraging the existing PGM hardware for quantification of non-glucose target
using different recognition molecules. The technology can be used to develop
new sensing platform using the technologies developed for the PGM over the last
decades.