(615d) Utilizing an Endogenous Pathway for 1-Butanol Production in Saccharomyces Cerevisiae Conference: AIChE Annual MeetingYear: 2014Proceeding: 2014 AIChE Annual MeetingGroup: Food, Pharmaceutical & Bioengineering DivisionSession: Biocatalysis and Biosynthesis II: Biofuels and Commodity Chemicals Applications Time: Thursday, November 20, 2014 - 9:24am-9:42am Authors: Si, T., University of Illinois at Urbana Champaign Luo, Y., University of Illinois at Urbana-Champaign Xiao, H., University of Illinois at Urbana Champaign Zhao, H., University of Illinois at Urbana Champaign Microbial production of higher alcohols from renewable feedstock has attracted intensive attention owing to its potential as a source for next-generation gasoline substitutes. Here we report the discovery, characterization and engineering of an endogenous 1-butanol pathway in Saccharomyces cerevisiae. Upon introduction of a single gene deletion adh1Δ, S. cerevisiae was able to accumulate more than 120 mg/L 1-butanol from glucose in rich medium. Precursor feeding, 13C-isotope labeling and gene deletion experiments demonstrated that the endogenous 1-butanol production was dependent on catabolism of threonine in a manner similar to fusel alcohol production by the Ehrlich pathway. Specifically, the leucine biosynthesis pathway was engaged in the conversion of key 2-keto acid intermediates. Overexpression of the pathway enzymes and elimination of competing pathways achieved the highest reported 1-butanol titer in S. cerevisiae (243 mg/L).