(420g) A Biofabrication Approach for Controlled Synthesis of Silver Nanoparticles with High Catalytic and Antibacterial Activities

Authors: 
Yi, H., Tufts University
Jung, S., Tufts University
Yang, C., Tufts University

Simple, facile and size-controllable synthesis of uniform Ag nanoparticles with tobacco mosaic virus (TMV) as a biomediator was developed in the absence of external reducing agents. Ag nanoparticles with average diameter of 2, 4 and 9 nm were obtained by simply tuning the ratio of TMV/Ag(NH3)2+. Autocatalytic growth followed by coalescence was observed from Ag formation in the presence of TMV.  The as-prepared TMV-mediated Ag nanoparticles show substantially higher catalytic and antibacterial activities than previous results. For the 4-nitrophenol hydrogenation reaction, the rate constants per surface area for 2 and 9 nm Ag nanoparticles were determined to be 0.64 and 1.2 L m−2 s−1 respectively. Both Kirby–Bauer disk diffusion test and tube culture results demonstrate high antibacterial activity of TMV-mediated Ag particles against Escherichia coli, with minimal inhibition concentration (MIC) of 2.3 and 2.5 ppm for 2 and 9 nm Ag nanoparticles respectively. We expect that our biomediated Ag synthesis approach can be readily extended to other biomaterials and metal nanoparticle systems.