(365f) Lipid Production from Lignocellulosic Biomass Via a Newly Isolated Oleaginous Fungal Strain Conference: AIChE Annual MeetingYear: 2014Proceeding: 2014 AIChE Annual MeetingGroup: 2014 International Congress on Energy (ICE)Session: Biochemical Conversion Processes in Forest/Plant Biorefineries III Time: Tuesday, November 18, 2014 - 5:20pm-5:45pm Authors: Yang, Y., University of Minnesota Yan, M., University of Minnesota Gan, J., University of Minnesota Rajendran, A., University of Minnesota Lin, H., University of Minnesota He, Q., University of Minnesota Hu, B., University of Minnesota Microbial lipids can be an attractive feedstock for biodiesel production to replace the current plant-oil based biodiesel. Oleaginous fungi have significant advantages, such as high growth rate and high oil production, easy harvest, good tolerance on growth conditions (e.g., inhibitors, pH), and can be easily grown and controlled in bioreactors. Several oleaginous yeast and fungal strains are capable of accumulating high amount of lipids inside cells, but rarely can directly utilize lignocellulosic biomass as substrates since the lack of cellulase producing capability. A newly isolated Fusarium strain from the endophytic fungi of soybean plants was discovered to generate high content of lipids (53.6-57.8% of dry biomass) and also a significant amount of cellulase enzyme compared to well-studied oleaginous strain Mortierella isabellina. This research focus on the growth behavior and characteristics of this new strain and the developing of different bioconversion processes utilizing lignocellulosic biomass for improved microbial lipid accumulation. The strain showed good carbon source utilization since it can produce a significant amount of lipids on most of carbon sources including glucose, xylose, mannose, galactose, glycerol, lactose, fructose, sucrose, cellobiose, starch, xylan, and cellulose. The strain cultivation (growth medium, temperature, agitation, light effect, etc.) was optimized and the extracellular cellulase production of this strain was enhanced by directed evolution. Investigation on the effect of pretreatment and cellulase enzyme were also conducted to achieve higher efficiency of converting lignocellulosic biomass to lipids.