(808e) A Green Approach to Mitigate CO2 Using Mixed Microbial Culture

Raghuvanshi, S., Birla Institute of Technology and Science (BITS) - BITS Pilani, Pilani Campus, Rajasthan, India
Gupta, S., Birla Institute of Technology & Science, Pilani
Mishra, S., Birla Institute of Technology & Science (BITS)

Carbon dioxide is one of the GHG’s (Green House Gases) & responsible for Global warming.. As of July 2011, CO2 in the earth's atmosphere is at a concentration of 392 ppm by volume which is 35% higher as compared to the mid-1800s. Carbon dioxide, which is estimated to contribute 50-60% of the Global warming in the last century, is majorly released during the combustion of fossil fuel. The CO2 concentration in earth atmosphere can be reduced by biological, chemical and technological techniques available through either mitigating or sequestering emissions. In the recent years, biological route has been identified as one of the most viable options available for the mitigation of CO2. There are six carbon fixing pathway’s.  Out of which, the Calvin cycle (Calvin-Benson-Bassham) is used by photoautotroph’s to fix CO2. There are microbes which are ubiquitous in nature and thrive in extreme environment classified as chemoautotrops. They also classified as Extremophiles. The requirement of industries is to develop a economic, efficient and effective system which not only fix CO2 but also capable to withstand in the severe industrial environment. Mixed culture is proved to be a superior candidate, as the consortium of different engineered species develop a symbiotic relationship which helps each other not only to survive in harsh conditions but also curb down CO2. The another possibilities are simultaneous waste water treatment, production of Methane,  and bio-hydrogen production. The present work deals in identification of microbes present in sludge of waste water treatment plant having CO2 fixation capabilities. It also gives the insight of  the relation which exists between natural selected mixed culture and to understand the capabilities of CO2 fixation. The batch study has been conducted to measure the capabilities of mixed culture to mitigate CO2 for five different V/V % concentration of CO2 4, 12, 16, 22 and 25. It was observed that CO2 is completed consumed by microorganisms in 4 days. Mitigation rate for different concentration of CO2, bio-mass growth rate and resulting product has been analyzed on per hour basis and per day basis. Mixed culture is found to be superior candidate than photoautotrophic organisms for CO2 mitigation. They show a promising future to develop a low cost, compact size closed system for in-situ CO2 mitigation.


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