(804h) A Smart Phone Fluorescent Basic Metabolic Panel

Mobile, point-of-care diagnostics can be used to more efficiently and effectively deliver health care both in and outside the hospital.  We have developed fluorescent particle sensors in combination with a handheld detection device and demonstrate the potential as a diagnostic system through measuring the complete basic metabolic panel.  Miniaturization of the particle sensors to the nanoscale reduces the volume of needed sample and increases the response time.  Fluorescence detection eliminates the need for electrical connection to the sensors and can be used easily with improving optical systems. The fluorescence sensors are based on optode technology and produce dual wavelength emission for quantitative measurements. Sensors to directly measure each of the ions in the panel were developed.  Biosensors for the small molecules in the panel were created through a combination of optode particle sensors and enzymes specific for the small molecule of interest.  The response of the sensors was used to measure the concentration of the basic metabolic panel components with a custom iPhone case that converts the phone into a dual wavelength fluorescence imager.  Future development of this system could be used for a simple point-of-care diagnostic device.