(548c) Process Development Comparison of Single Use Clarification Technologies for Mammalian Cell Cultures Utilizing a Conventional Two-Stage Depth Filtration, a New Optimized Depth Filter and a Novel Single-Use Fluidized Bed Centrifuge With Depth Filtration | AIChE

(548c) Process Development Comparison of Single Use Clarification Technologies for Mammalian Cell Cultures Utilizing a Conventional Two-Stage Depth Filtration, a New Optimized Depth Filter and a Novel Single-Use Fluidized Bed Centrifuge With Depth Filtration

Authors 

Castano, J. C. - Presenter, EMD Millipore Corp.
Westoby, M. M., Biogen Idec
Elouafiq, S., Biogen Idec
McCann, D., Biogen Idec



Single-use cell culture harvest technologies have been challenged considerably by cell culture development leading to higher productivity and cell densities with equivalent increased impurities and debris. 

Conventional two-stage adsorptive depth filtration is currently used as a single use strategy for cell culture clarification, however, high density cell culture processes and increased batch volumes can impact depth filtration performance and render facility fit limitations. 

A collective evaluation with multiple antibodies was accomplished to make a direct comparison of mammalian cell cultures at native and low pH (acid precipitate to induce flocculation) conditions utilizing a conventional two-stage depth filtration,  two-stage single-use centrifuge with fluidized bed coupled with depth filtration and single-stage new optimized depth filtration. 

This presentation will compare the results for each technology based on their performance at clarifying high-density cell cultures, reduction of impurities, recovery, and depth filtration to depict an optimal and feasible solution for single-use harvesting of high density cell cultures.