(481a) Investigation of a Novel Platform for Manipulation of Microparticles Using Dielectrophoresis

Investigation
of a Novel Platform for Manipulation of Microparticles
using Dielectrophoresis

Stephanie Angione¹, Derek Croote¹, Sara Karlberg²,
Anubhav Tripathi¹

¹Center for Biomedical Engineering, School of Engineering and
Division of Biology and Medicine, Brown University, Providence, RI

² School of Biotechnology, KTH Royal Institute of Technology,
Stockholm, Sweden

Introduction:

Dielectrophoresis (DEP) is a commonly
used tool for the study and manipulation of micron and sub- micron particles,
including biological molecules, in solution. Because biological molecules of
interest are often found at concentrations so low that immediate analysis by
current methods is not possible, pre-concentration is necessary as a prior step.
The field of diagnostics is plagued by the use of off-chip pre-concentration
methods, which prevents real-time testing in resource-poor settings at the
point of care. Current DEP platforms do not address the needs of diagnostic
applications where pre-concentration of sample must be completed in a rapid and
simple manner.  Our novel platform, is a potential solution to this problem as it
combines the established science of DEP for the manipulation of DNA, proteins
and cells with a unique electrode material and geometry.

Materials
and Methods:

Polystyrene microparticles
(FluoSpheres, Invitrogen) were characterized by the
manufacturer as being 9.9 ±0.1188 µm in diameter, with a density of 1060 kg/m³.
The stock concentration was given as 3.6 x 10⁶ beads/mL, and further
diluted in 1000 µl of the appropriate conductivity KCl
solution. Experiments were performed on a Nikon Eclipse TE2000 inverted
microscope equipped with a Pixelink PL-B681 CMOS
camera for image and video acquisition. The polydimethylsiloxane
(PDMS) microchip containing micro-channels and reservoirs was designed to allow
for imbedding of the pipette tips within the design. PDMS microchip fabrication
was done using standard photolithography procedures.

Results
and Discussion:

We present the validation of an
innovative electrode material and PDMS microchannel geometry for dielectrophoretic concentration of 10 µm polystyrene beads.
We have implemented carbon black compounded polypropylene pipette tips as
viable electrodes for the generation of electric fields in solution that can be
implemented to concentrate and target particles within the pipette tips for
easy removal. We have performed modeling and experimental validation of the
particle movement, which indicates that microparticle
behavior is a function of conductivity and frequency. The observed relationship
of particle velocity to medium conductivity and applied electric field
frequency has practical implications for particle manipulation using conductive
pipette tips. To accomplish successful trapping, the particle velocities were
maximized and Figure 1 displays the microparticle
velocity as a function of frequency and conductivity, indicating that the 5x10^-6
S/m conductivity solution with an applied electric field frequency of 10⁵
Hz was optimal for increasing the particle motion. This platform provides an
innovative way to improve diagnostic techniques and sensitivity by targeting,
trapping and concentrating particles.

Conclusion:

The aim of this work was to create a
platform to utilize and validate carbon black compounded polypropylene pipette
tips as viable electrodes for the generation of electric fields in solution.
The use of this innovative electrode material for dielectrophoresis of 10 µm
polystyrene particles was established as a proof of concept for
pre-concentration, trapping and targeting of particles. We characterized the
behavior of microparticles in solutions of varying
conductivities under the effects of applied electric fields of varying
frequencies to optimize particle motion and trapping. Further work will focus
on concentrating and trapping DNA from dilute solutions for direct
amplification and detection using nucleic acid amplification methods.