(107h) Microbial Production of Renewable Monoethylene Glycol | AIChE

(107h) Microbial Production of Renewable Monoethylene Glycol


Pereira, B. - Presenter, Massachusetts Institute of Technology
De Mey, M., Ghent University
Zhang, H., Massachusetts Institute of Technology
Stephanopoulos, G., Massachusetts Institute of Technology
Lim, C. G., University at Buffalo, the State University of New York

Monoethylene glycol (MEG) is an important commodity chemical which is utilized in such applications as plastic packaging, polyester fabric, and antifreeze.  It is derived from fossil fuels, and therefore, to reduce its environmental impact, we propose a single-step bioprocess in which plant-derived carbohydrates are converted by engineered microorganisms into renewable MEG.  Toward this goal, we have engineered novel metabolic pathways for the biological production of MEG into strains of E. coli.  In this work, we validate these pathways and demonstrate the production of MEG from various sugars, achieving titers up to 40 g/L at yields of 0.35 g-MEG/g-xylose.