(789d) Spatio-Temporal Cell Micro-Patterning with Photo-Cleavable Poly(ethyleneglycol)-Lipid

Photo-regulated micro-patterning of living cells is a promising technique for tissue engineering and cell microarray. However, all reported methods are based on cellular adhesiveness. This is why conventional patterning methods are not applicable to non-adherent cells, which include many biologically and medicinally important cells such as blood cells and a part of cancer and stem cells. The objective of this study is to expand the application range of a micro-patterning technique. We have previously reported that any types of cells can be effectively immobilized on the surfaces modified with PEG-lipids. In this study, we newly synthesized a photo-cleavable PEG-lipid by incorporating a photo-cleavable linker between the PEG and lipid units. This PEG-lipid was successively confirmed to be digested by irradiation of UV light with wave length of 365 nm. When UV light above 1 J/cm² was irradiated on the surface modified PEG-lipid, cells didn’t attach on the irradiated area, whereas cells attached on the unirradiated area. Furthermore, the micro-patterns of any types of cells such as dot, line and symbolic patterns were easily prepared by irradiating UV light on PEG-lipid modified surfaces. In addition, the patterned cells were confirmed to be alive and be abele to be cultured. Furthermore, the targeted cell spots of a cell microarray were specifically released from the substrate by irradiating some spots of UV lights. Thus, the present photo-cleavable PEG-lipid is useful for spatio-temporally manipulating any types of cells on the substrate and is a promising tool for cell sorting on a single cell array after high-content cell analysis.