(682b) Combination of Spectroscopic Methods for Inline Monitoring and Control of Mammalian Cell Cultivations

Bioprocesses are complex systems and the need of their monitoring through PAT tools has long been an integral part of industrial processes since it allows increased automation and introducing of advanced control schemes.

The integration of strengths of different spectroscopic methods such as Near Infrared spectroscopy, Mid-Infrared spectroscopy, 2-Dimensional Fluorescence Spectroscopy and Dielectric Spectroscopy allowed monitoring and control of different culture attributes to be readily available (inline) instead of being determined in the laboratory (offline). This is an advantage over analytical laboratory methods, since spectroscopic sensors can enable the intensification of in-process analytics.

Moreover, since these spectroscopy’s are focusing on information of different quality attributes (chemical, physical, physiological) extractable from a biotechnological cultivation process, the combination of those has the potential to enhance the process understanding significantly. An overview of our experience in the integration of above techniques - to establish process knowledge in a reduced number of cultivations for different media, clones and products - will be described.