(596n) Scaffold Fabrication From Cadaver Goat Lung Tissue and Its Biocompatibility Study for Tissue Engineering Applications

Mishra, N. C., Indian Institute of Technology Roorkee, Roorkee,India
Gupta, S., Indian Institute of Technology Roorkee
Dinda, A. K., All India Institute Of Medical Sciences

Fabricating scaffold from cadaver tissue and its application to regenerate various tissues is a current challenge in the area of tissue engineering. Our objective is to fabricate scaffold from cadaver goat lung, and to test its biocompatibility for application in tissue engineering. Scaffold was fabricated by decellularizing cadaver goat-lung by physical and chemical methods, and characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD) and energy dispersion spectroscopy (EDS). Cell viability was performed by MTT assay after 24 hours in culture, by seeding Hep G2 cell lines onto the scaffold. Gross morphology of decellularized and recellularized lung tissue scaffold was demonstrated by Hemotoxylin and Eosin staining.  SEM images of decellularized lung revealed porous interconnected structure as expected for a lung scaffold. XRD study indicates the amorphous nature of the scaffold matrix and EDS study proves the composition of the scaffold to be organic.  MTT assay resulted in 99.2% viability of the cells in the scaffold. The histological examination revealed no remaining cells in the decellularized lung-tissue scaffold with negative cell nuclei staining, but nuclear counterstaining was detectable in the recellularized lung scaffold. The results demonstrated a novel butt simple and economically feasible method of decellularization of cadaver lung. The biomimetic nature of the scaffold and high cell viability in the scaffold, shows its potential to be a good candidate for tissue engineering application and for studying cellular responses for drug discovery.