(409b) Using a Recombined Microorganism Consortium for One-Step Production of Ethanol and Hydrogen Directly From Cellulose

Consolidated bioprocess (CBP) is referred to use a super strain to realize one-step cellulosic ethanol production. It has been proven to be impossible in a short term by many literatures.  The utility of multifunctional reconstructive consortium referred by our research team is a promising solution to overcome this bottleneck.  Consortium H which can convert cellulose into ethanol and hydrogen under anaerobic fermentation was isolated from farm soils in Shandong Province, China. Construction information of consortium H was analyzed by Denaturing Gradient Gel Electrophoresis (DGGE) and Terminal Restriction Fragment Length Polymorphism (T-RFLP). Based on these data, strains with outstanding capability of conversion of cellulosic ethanol were isolated from consortium H. A new consortium reconstructed by the selected strains and the original consortium H was found to significantly increase ethanol conversion rate and yield of hydrogen production. Its ethanol conversion rate was increased from 61% to 81%, while the highest hydrogen production rate of this consortium was increased from 200mL/day L culture to around 240mL/day L culture. T-RFLP was used to monitor the construction changes of this reconstructive consortium, and the results showed obvious changes between the new consortium and consortium H during fermentation process. Further investigation relating to mechanism will be performed on consortium construction and metabolism flux based on metagenomics and metatranscriptomics sequencing. Our group named this method which to reconstruct a novel consortium consulted by bioinformatics as constituted biology, and a series of experiments on how to realize precise regulation on consortium based on this method are being held.