(276f) Characterization of Lysozyme/Single-Walled Carbon Nanotube/ Polyvinyl Alcohol Films

Through the integration of lysozyme (LSZ) and single-walled carbon nanotubes (SWNT), a robust film coating was developed that could potentially be placed in public environments and reduce the transfer of communicable illness. LSZ, an enzyme found in egg whites, tears, and saliva, possesses antibacterial characteristics but lacks mechanical strength. Previous research has shown that SWNT can provide strength to lysozyme films and coatings without hindering antibacterial properties. Coatings made by layer-by-layer assembly without any polymer binder possessed high mechanical strength but limited wear and solvent resistance. To increase mechanical robustness, polyvinyl alcohol (PVA) was incorporated with the LSZ-SWNT dispersions. The resulting films were characterized for their electrical, mechanical, optical, thermal properties and antibacterial activity. Films were produced from both concentrated bulk mixtures and supernatant dispersion.  Both types of films lacked electrical conductivity, but maintained antibacterial activity.  Mechanical testing showed that the supernatant films were stronger; this is likely the result of improved dispersion. To evaluate the films’ durability in various environments, solvent rub testing involving different acidic and basic solutions were conducted. These results demonstrated the versatility of the films’ potential applications.