(744d) Nanomagnetic Multiplex Biology Assay and High THROUGHPUT Biosensing Instrument

Bioassays that detect and quantify biomolecules at ultra-low quantity, are of great need in biomedical engineering. Spintronic sensors, such as giant magnetoresistive (GMR) sensor, have emerged as one promising platform for biomolecule detection because of its sensitivity, matrix-insensitive, low-cost and ease-to use. We herein report the development of a novel detection system based on highly sensitive GMR sensor and sub-13nm high-magnetic-moment FeCo nanoparticles, and the usefulness of this system for quantificating protein markers with extremely low concentration down to 150 copies per sensor surface (~10 fM sensitivity) within 5 mins. Magnetic label with high magnetic moment is preferred for the sake of generating larger magnetic signal, thereby higher sensitivity for the entire detection system. Furthermore, micro and sub-micro sized magnetic labels are not favorable for biomolecule detection due to the low diffusivity and poor binding selectivity. We have successfully demonstrated the application of sub-13 nm high moment FeCo magnetic nanoparticles with GMR biosensor for the first time. Furthermore, we will report a bench-top biosensing instrument and the developed nanomagnetic multipex protein-assay, 64-sensor per reaction well (5 mm in diameter), to directly quantify multiple proteins simultanuously and also to monitor and characterize the binding dynamic of the protein with different binding surfaces/sites.