(3ci) Immuno-Liposome Nanoparticles for Single Cell Array

Immuno-Liposome Nanoparticles for Single Cell Array

Kwang Joo Kwak^1,2

¹NSF Nanoscale Science and Engineering
Center for Affordable Nanoengineering of Polymeric Biomedical Device
(NSEC-CANPBD)

²Department of Chemical and Biomolecular
Engineering

The Ohio State
University, Columbus, Ohio 43210

*Contact author-
E-mail: kwak.63@osu.edu

Recent advances in biomimetic lipid
membranes provide opportunities to develop applications such as biosensing, gene/drug
delivery, and cellular recognition. My research interests are focused on tethered
immuno-liposome nanoparticles (t-ILNs) that can be conjugated on a planar
surface through a self-assembled monolayer of a thiolipid and modified with cell
adhesion molecules and therapeutic targeting antibodies. The multi-antibody
conjugated t-ILNs have been investigated to be able to isolate extremely rare
cells such as cancer stem cells from the whole human blood. The t-ILNs array
based on a centrifugal force has been demonstrated to improve sensitivity and
specificity of stem cell isolation by increasing the focal adhesion between cell
and the ILN surface and preventing non-specific binding on the interfaces. Novel t-ILN array based cell isolation
has higher potential for many other important biomedical applications such as
prognosis of cancer patients and monitoring targeted therapy.