(86g) Study Cancer Cell Migration Phenomenon Utilizing a Microfluidic Device Consisting Microgaps with Different Gap Size | AIChE

(86g) Study Cancer Cell Migration Phenomenon Utilizing a Microfluidic Device Consisting Microgaps with Different Gap Size

Authors 

Tong, Z. - Presenter, Johns Hopkins University
Dallas, M. - Presenter, Johns Hopkins University
Hung, W. - Presenter, Johns Hopkins University
Stebe, K. J. - Presenter, University of Pennsylvania
Konstantopoulos, K. - Presenter, Johns Hopkins University


Cell migration is an important physiological as well as pathological process, including cancer metastasis. Once a tumor cell dislodges and releases to blood stream, it undergoes sequential steps of adhesion, deformation, and transmigration through endothelial lining and basement membrane, which was believed to be guided by chemokine secretion from distant organs. To further investigate this form of guided cell migration we fabricated a microfludic device capable of maintaining stable gradient of chemo-attractant, such as FBS or CXCR4. Moreover, an array of microgap in the range of 3 μm to 50 μm within the microfludic chamber was used to study the gap size effect on cancer cell migration. Our preliminary data showed that when micro-channel gap size is smaller than 5 μm, Human Osteosarcoma Cell (HOS) was not able to deform and migrate through the microchannels. The experimental setup was conducted over a live cell chamber and we were able to quantify the migratory and deformability of a single cell using direct imaging. Moreover, we would like to quantify the effect of different ECM coated surfaces on cancer cell migration. We were particularly interested to study the high level CD44 expressing HOS cells on binding to Hyaluronic Acid (HA) coated substrate, as HA contributes to cell movement, proliferation, and tumor growth. CD44 knock-down HOS cells were generated using siRNA technology to access the effect of depletion of CD44 molecule on cancer cell migration and growth on HA coated surface.