(548c) “One-Pot” Synthesis of Lipase Nanogel | AIChE

(548c) “One-Pot” Synthesis of Lipase Nanogel

Authors 

Xu, D. - Presenter, Tsinghua University
Lu, D. - Presenter, Tsinghua University
Liu, Z. - Presenter, Key Lab of Industrial Biocatalysis, Ministry of Education, Tsinghua University


Lipase is one of the most extensively applied enzymes and plays important role in baking, laundry detergents, production of biofuel and etc. It is proposed by Liu and his coworkers that, once encapsulated into a polyacrylamide nanogel, Candida rugosa lipase (CRL), is granted a significantly improved stability at both at high temperature and in the presence of organic solvent. This enhanced stability, which is obtained without compromise of the catalytic activity, could thus enable an extended spectrum of lipase catalysis, particularly those conducted at harsh conditions found in most industrial processes. The present study described a ?one-pot? synthesis of CRL nanogel, using GMA for the acryloylation of lipase while the acrylamide as the monomer. Compared with the established aqueous two-step in situ polymerization, this new procedure yielded lipase nanogel with further improved thermal stability. This new and straightforward synthetic method might also be applied for the fabrication of other enzymes for industrial catalysis.

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