(262c) Direct Electrochemistry and Electrocatalysis of Electrospun Single-Walled Carbon Nanotubes-Hemoglobin Composite Microbelts – towards the Development of Sensitive and Mediator-Free Biosensor

Single-walled carbon nanotubes (SWNTs) and hemoglobin (Hb) were co-electrospun to generate SWNTs-Hb microbelts, a novel composite material combining the advantages of excellent electron transfer property of SWNTs, electrocatalytic capability of redox Hb proteins, and highly porous structure of microbelts. FT-IR spectra confirmed that Hb in the microbelts kept its native conformational structure and Raman spectra demonstrated the successful incorporation of SWNTs in the microbelts. The direct electrochemistry of SWNTs-Hb composite microbelts was investigated and its application for electrocatalytic reductions and sensitive detections of three compounds, hydrogen peroxide, nitrite, and trichloroacetic acid, was also conducted. The comparison of the SWNTs-Hb composite microbelts modified electrode with the Hb microbelts modified electrode and other reported electrodes in the literature indicates that the incorporation of SWNTs into Hb microbelts can significantly enhance the direct electrochemistry of Hb and the SWNTs-Hb microbelts based biosensor has great potential application in the mediator-free detection of various analytes.