We are aware of an issue with certificate availability and are working diligently with the vendor to resolve. The vendor has indicated that, while users are unable to directly access their certificates, results are still being stored. Certificates will be available once the issue is resolved. Thank you for your patience.

(137b) Directing Cell Motions On Micro-Patterned Surfaces

Mahmud, G., Northwestern University
Soh, S., Northwestern University
Huda, S., Northwestern University
Kandere-Grzybowska, K., Northwestern University
Grzybowski, B., Northwestern University

Cell motility is a process deriving from the synchronized dynamics of the cytoskeleton. In several important physiological processes?notably, cancer metastasis?the randomly moving cells can acquire a directional motility phenotype and bias their motions in response to environmental cues. Despite intense research, however, the current understanding of directional cell migration is incomplete and there is a growing need to develop systems that would enable the study and control of this process. This article demonstrates that random motions of motile cells can be rectified by asymmetric ('ratchet') microgeometries. Interactions between the cells and the imposed geometrical cues guide cell polarization and give rise to directional motility. Depending on the ratchet design, cells of different types can move either in the same or in opposite directions on the same imposed pattern. In the latter case, it is possible to partially sort mixed cell populations into different collecting reservoirs.