(488t) Preparation, Characterization and in Vitro Skin Permeation of Solid Lipid Nanoparticle(SLN) Coating with Silk Protein

Solid lipid nanoparticles (SLN) have been turned out to be a versatile carrier. The nanoparticles were reported to accommodate triptolide, an anti-inflammatory agent, and enhance the transdermal delivery of the drug. It was also evaluated as a vector for a gene delivery. Following the results, it boosted in vitro transfection activity by optimizing composition of the cationic lipid and matrix lipid of SLN. In this study SLN composed of stearic acid, synthesized basic amino acid as a copolymer and silk protein. A copolymer of arginine and leucine acid was prepared by condensation reactions and pKb of the copolymer is 12.5. If the pH was lower than pKb, basic polypeptide was positive charge and stearic acid (SA) was negative charge in the pH above 4.5. Moreover, it is appropriate to choose this basic polypeptide as an emulsifier for SLN. SA and basic polypeptides were homogenized for 20min at 70 °C. The hot emulsion was carefully added to distilled ice water (0 ? 5 °C) containing silk protein. The cosmetic adapted with silk protein, which can be used to improve the moisture and antioxidant of skin. Furthermore, in order to get an idea of the feasibility of the cosmetic application, the in vitro skin permeation of SLN incorporated in the nanoparticles was investigated. The physical characteristics such as size distribution in alcoholic solution and stabilities against sodium lauryl sulfate (SLS), apparent shape, microelectrophoretic behavior were also investigated.