(488s) In Vitro Skin Permeation of Cubosomes Containing Water Soluble Extracts of Barberry

The MO/water system, cubic phase, can be used as a delivery vehicle for hydrophobic and/or hydrophilic materials. A wide variety of drug with different physicochemical properties have been incorporated in MO-based cubic phases for stabilization and sustained release. Monoolein(MO) cubic phases containing water soluble extract (WSE) from Berberis koreana (Korean barbbery) were prepared by hydrating the molten MO with aqueous solutions of WSE (0.5 %, 1.0%, 1.5 %). To obtain the nanoparticles of cubic phase(so called cubosomes), Pluronic F127 solution in the buffer was added to MO gel so that the ratio of the polymeric surfactant to MO was 15:100. And then the gel were micronized in a bath type sonicator at room temperature for 15min. In vitro skin permeation of WSE entrapped in the cubosomes was investigated using a diffusion cell drive. As a control, WSE dissolved in phosphate-buffered saline (10mM, pH 7.4) was used. According to the results, cubosomes enhanced the skin permeation. The release of WSE from the cubic phase seemed to be a first order pattern When compared with WSE solution in PBS(10mM, pH 7.4). In order to cubic phase transition temperature of cubic phase containing WSE, determined using DSC. In addition, the physical characteristics such as size distribution and shape were observed dynamic light scattering(DLS) and transmittance electron microscopy(TEM).