(440e) N-Butanol Production Via Fermentation of Sugar Maple Wood Extract Hydrolysate

Authors: 
Sun, Z., SUNY College of Environmental Science and Forestry
Mukherjee, S. S., SUNY College of Environmental Science and Forestry
Fitzgerald, L., SUNY College of Environmental Science and Forestry
Liu, S., SUNY College of Environmental Science and Forestry


In this study, the production of n-butanol via fermentation of sugar maple hemicellulose hydrolysate, using Clostridium Acetobutylicum ATCC824 and Clostridium Beijerinckii ATCC 35702 were investigated. When using static pH-uncontrolled fermentation in untreated hydrolysate, ATCC 824 only produced 0.2g/L butanol. Over -liming and increasing osmotic pressure of hydrolysate with added phosphate salt significantly increased butanol production from 0.2 to 2.4g/L. However using dynamic pH-controlled stirred tank reactor significantly improved the butanol production of ATCC 824 from the concentrated hydrolysate without treatment. After 96 hours fermentation 53 g/L reducing sugar was consumed and 7 g/L butanol, 2.64g/L acetone and 0.81g/L ethanol were produced by C. acetobutylicum ATCC824. For C. Beijerinckii ATCC 35702 in untreated hydrolysate and at first 48 hours, cells utilized about 35 g/L reducing sugar and produced 4.65 g/L butanol, 2.11g/L ethanol and 2.27g/L acetone. Unfortunately after 48 hours ATCC 35702 stopped growing and utilizing substrate.

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