(160a) Enhanced Resistance of DNA Nanostructures to Enzymatic Digestion
AIChE Annual Meeting
2009 Annual Meeting
Materials Engineering and Sciences Division
Monday, November 9, 2009 - 3:15pm to 3:35pm
The ability of enzymes to bind their substrates, and to perform catalytic functions, depends on environmental conditions and molecular recognition. Due to their size and shape, discrete DNA nanostructures might have unusual properties when compared to linear DNA. We report the enhanced resistance of a DNA tetrahedron to the action of several nucleases, both specific and non-specific. Most dramatically, the DNA tetrahedron is substantially more stable than its linear counterparts in the presence of 10% fetal bovine serum at physiological temperature. A tentative explanation, based on the properties of DNA and the enzymes studied, is presented. Designs of DNA nanostructures intended for biological applications will therefore require additional considerations. We are currently exploring the possibility of a nanostructure-based approach to antisense gene knockdown.