(485j) Nanofluidic T-Junctions for Single Molecule DNA Mapping

Authors: 
Tang, J. - Presenter, Massachusetts Institute of Technology


Controlled trapping and stretching of DNA molecules is critical for single molecule genomic and polymer physics studies. To date, most devices are based on hydrodynamic stretching of DNA. In contrast, we exploit the fact that DNA is a homogeneously charged polyelectrolyte and so will migrate in an electric field. Here we present T-junction and cross-slot devices which can trap and stretch DNA using electric field gradients. The devices do not require special end-functionalization of the DNA. The purely elongational nature of the electric field allows us to use thin nanofluidic channels. We show that two physical mechanisms of stretching can occur depending on the length of the DNA relative to the channel width in the junction region. In one case the governing dimensionless group is a Deborah number and in the other a Peclet number. Stable trapping and stretching of DNA molecules up to lengths of 485 kilobasepairs is demonstrated. Applications in single molecule mapping will also be demonstrated.