(350a) Proteomic Analysis of Cardiomyocytes Following Treatment with Soy-Derived Phytoestrogens | AIChE

(350a) Proteomic Analysis of Cardiomyocytes Following Treatment with Soy-Derived Phytoestrogens

Authors 

Sun, Z. - Presenter, Colorado State University
Hamilton, K. L. - Presenter, Colorado State University
Reardon, K. F. - Presenter, Colorado State University


Epidemiological observations comparing populations from different ethnic groups and countries suggest that higher consumption of soy-containing foods contributes to the lower incidence of cardiovascular disease in the Asian population than in the American population. Two major phytoestrogens, genistein and daidzein, which constitute the major part of the total isoflavones in soy protein respectively, are thought to have a potential role in improving risk factors for cardiovascular disease. Although phytoestrogens are generally accepted as beneficial for the cardiovascular system, scientific certainty about the relationship between soy and cardiovascular diseases is still lacking. Several studies have suggested that soy-derived phytoestrogens may potentially exert their effects in cardiomyocytes via many means including estrogen receptor-dependent and independent mechanisms. Phytoestrogens were also reported to have other cellular effects including inhibition of cell cycle progression, inhibition of angiogenesis, attenuation of oxidant damage, kinase modulation, and regulation of growth factors. Moreover, the biological activity of soy-derived phytoestrogens is likely to vary substantially in dose- and cell type-specific manners. Due to this complex nature of cellular responses to phytoestrogens, a more powerful approach to evaluate the mechanism systematically is needed.

The overall goal of this research is to investigate changes in the proteome of heart tissue following exposure with genistein and daidzein (10-100 µM). Our first study has focused on cultured rat cardiomyocytes as a model system. 2D electrophoresis has been employed to compare the protein profiles of treated and control samples, and differentially expressed proteins have been analyzed by mass spectrometry. More than fifty proteins were shown to be differentially expressed when cardiomyocytes were treated with 10µM or 50µM of genistein, including voltage-dependent anion-selective channel protein 2 and heat shock proteins. These and other results will be discussed, along with experiments in which HILIC-RP LC-MS based shotgun approach is used to target membrane-associated proteins.