(178d) Use of Cfd to Identify and Resolve Cell Culture Issues Observed at Production Scale during Technology Transfer
AIChE Annual Meeting
2008
2008 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Scale-up Issues in Pharmaceutical Formulation Development
Monday, November 17, 2008 - 4:15pm to 4:35pm
Substantial experimental work has been dedicated to correlating animal cell culture performance to cell culture process conditions. In this work we present a numerical case study on the environment to which cells are exposed in mechanically agitated and sparged bioreactors. For two modeled production scale bioreactors with different designs and process conditions, we compare mechanical shearing force, Reynolds stress, power input per volume, turbulence energy dissipation rate, detrimental Kolmogorov length scale distribution, as well as bubble residence time in impeller zones and tanks. Detailed and complete information was helpful to understand the culture process performance issues encountered during technology transfer, and to consequently identify an optimal operating parameter design space.