(112c) Enzymatic Synthesis of Enantiomerically Pure Alcohols in Two-Phase Systems

Authors: 
Bangasser, B. L., South Dakota School of Mines and Technology
Mueller, P., RWTH Aachen
Greiner, L., RWTH Aachen


Enantiomerically pure small alcohols are useful as buidling blocks for pharmaceuticals and fine chemicals. These alcohols can be synthesized from prochiral ketones using chemical and biocatalysis. A greater enantiomeric excess (ee) can be achieved through biocatalysis, specifically with the enzyme alcohol dehydrogenase (ADH). Using ADH, two-phase reaction systems containing an aqueous reactive phase and an organic reservoir phase provide superior reaction conditions compared to one-phase systems. The presented research examines the synthesis of (R)-2-butanol in both batchwise and continuous two-phase biocatalyzed systems. The greatest batchwise ee achieved was 96% while gaining a 69% conversion. Employing a continuous reaction system, the greatest ee was 93% with a conversion of 68%.