(520f) Optimization of Medium Constituents for the Production of L-Glutamic Acid by Brevibacterium Roseum Using Response Surface Methodology

Authors: 
Nikku, M. Y., Andhra University
Donthireddy, S. R. R., Andhra University
Chaduvula, A. I. R., Andhra University, Visakhapatnam, AP, India
Uppuluri, K. B., Bapatla Engineering College
Imandi, S. B., Center for Biotechnology
Nalla, K. K., Andhra University
Dasari, V. R. R. K., Center for Biotechnology


Abstract

Statistical experimental design was used to optimize the conditions viz. Ethanol, Ammonium phosphate and Biotin concentration of L-Glutamic acid from Brevibacterium roseum NCIM 2238 by submerged fermentation. Maximum L-Glutamic acid concentration of 53.01621 g/l was obtained. The optimum conditions were found to be ethanol concentration of 5.3646 %v/v, ammonium phosphate of 0.7081 %w/v and biotin concentration of 1.6735 µg/ml. Glutamic acid production was also effectively promoted by optimizing the medium composition using Response Surface Methodology (RSM). Basically, this optimization process involves three major steps: performing the statistically designed experiments, estimating the coefficients in the mathematical model, and predicting the response and checking the adequacy of the model. Thus, by using SmF process with Brevibacterium roseum, the Central Composite Design (CCD) was found to be the most favorable strategy for investigating with respect to L-Glutamic acid production.