(515ai) Design And Simulation Of A Self Assembly Enzymatic Pathway Splitting Water Powered By Carbohydrate

Cell-free synthetic enzymatic pathway engineering can be regarded as the simplest synthetic biology example, which does not include gene expression regulation. Here we designed a synthetic enzymatic pathway consisting of 13 enzymes and one coenzyme for producing the theoretical yield hydrogen from starch and water as C6H10O5 (l) + 7 H2O (l) -> 12 H2 (g) + 6 CO2 (g) [PLOS ONE, DOI: 10.1371/journal.pone.0000456]. We are building a mathematical kinetic model that simulates the whole process. With help of the model, we can not only identify the rate-limiting steps for the whole process but also predict what key enzyme properties need improved and the potential maximum reaction rates. This new cell free synthetic biology technology could have broad applications and advantages, as compared to microbial conversion, because of its unique advantages such as, a theoretical yield, fast rate, and broad reaction conditions, etc.