(333i) Optimal Separation Times for Biomacromolecules in A Couette-Based/electrophoretic Separation Device: Effect of Capillary Wall Velocities
AIChE Annual Meeting
2007
2007 Annual Meeting
2007 Annual Meeting of the American Electrophoresis Society (AES)
Aes Poster Session
Tuesday, November 6, 2007 - 6:30pm to 8:30pm
The ratio of capillary wall velocities in an electrophoretic/Couette-based separation device is examined in this contribution. The flow within the device is modeled by the coupling electrostatics and hydrodynamics. In addition, the convective-diffusive differential model with the spatial averaging technique is used to predict the behavior of the system by obtaining analytical expressions for effective parameters (i.e. the convective velocity and universal dispersion) (Zanotti and Carbonell, 1984a, 1984b, 1984c). Previous analyses have yielded numerical solutions for a Poiseuille type of flow in a rectangular capillary channel (Sauer et. al., 1995). Furthermore, analytical solutions for a Poiseuille flow regime in a rectangular capillary channel were obtained by Oyanader and Arce (2005); it was also found in this contribution that the orthogonal field controlled the efficiency of separation in the device. Recently, Pascal et. al. (2007) applied the same approach to a Couette type of flow in a rectangular capillary channel with the top wall of the channel moving at velocity, vp, to study the effects of the cation valence, z, of two biomacromolecules. In this contribution, both walls of the capillary channel have velocities different from zero, and move in opposite directions to produce a Couette type of flow. By using the Peclet number as a parameter, the optimal time of separation has been computed for a range of values of the ratio of the channel wall velocities, R.
References:
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