(612c) Stem Cell Microarrays: Investigation of Factors That Influence Neural Stem Cell Fate
AIChE Annual Meeting
2006
2006 Annual Meeting
Food, Pharmaceutical & Bioengineering Division
Tissue Engineering: Stem Cells in Tissue Engineering (II)
Thursday, November 16, 2006 - 3:55pm to 4:15pm
We have developed a high throughput microarray-based approach for gene function screens in stem cells. Our microarrays are generated using soft lithographic microfabrication techniques; the method can be used for both loss and gain of function screens, and is compatible with both adult and embryonic stem cells. The method allows us to assay in parallel various cell functions including signaling, differentiation, and proliferation. To demonstrate the utility of this approach, we have screened cDNA libraries for factors that affect adult neural stem cell function. Our method would facilitate genome-scale gene function studies in a variety of mammalian cells, as well as the identification of drug targets and the generation of therapies for neurodegenerative diseases.