(439b) Isolation, Amplification and Detection of Viral RNA

The poster presents a study on isolation of target sequences, quantifying binding efficiency, and calculations of minimum viral load density needed in the samples of synthetic, non-virulent vRNA sequences for human hemagglutinin (H) and neuraminidase (N) genes. DNA primer/promoters are created to capturing the vRNA by linking to beads for purification. Purified vRNA is converted to cDNA and amplified in a continuous flow reaction using a T7-polymerase. The rates of RNA synthesis, durability, amplification efficiency are evaluated. The synthesized sequences are evaluated with specifically designed molecular beacon sequences identifying a known human influenza subtypes H1N1. The hybridization specificity, reaction time and signal to noise ratios are evaluated.