(326e) Purification of Biomolecules by Multicolumn Countercurrent Solvent Gradient Chromatography (Mcsgp)

Müller-Späth, T., ETH Zürich
Aumann, L., ETH Zurich
Ströhlein, G., ChromaCon AG
Tarafder, A., University of Tennessee
Morbidelli, M., Institute of Chemical and Bioengineering, ETH Zurich

The MCSGP-technology1 represents a multicolumn chromatographic purification process that is suited for purification of single components from multi-component mixtures at higher yields, purities and productivities than batch chromatography. The increase in separation capability is due to the countercurrent movement of mobile and stationary phase that allows even components of similar selectivities to be separated. The process unit combines both elements from batch chromatography and SMB. Furthermore, in contrast to SMB, linear solvent gradients can be applied within the unit. Possible implementations include the purification of peptides and antibodies. The separation performance with respect to yield, purity and productivity can be controlled by changing operating parameters such as flow rates and solvent gradients. The impact of single-parameter variations of two flow rates and the switch time have been examined with respect to the purification of monoclonal antibodies. Experimental data and modelling results will be shown and the consequences of the performance control on yield, purity and productivity will be discussed.

1 A continuous, counter-current multi-column chromatographic process incorporating modifier gradients for ternary separations, Ströhlein G, Aumann L, Mazzotti M, Morbidelli M, submitted to J of Chrom A on 02/27/06