(213d) Development of Affordable Bioelectronic Interfaces Using Medically Relevant Soluble Enzymes

Bioelectronic interfaces which establish electrical communication between bioactive proteins and electrodes have potential applications in biosensors, biocatalytic reactors, and biological fuel cells. We have fabricated bioelectronic interfaces using secondary alcohol dehydrogenase (sADH), sorbitol dehydrogenase (SDH), and mannitol dehydrogenase (MDH) on gold electrodes. The interfaces can be developed into sensors for acetone, sorbitol, histamine, and fructose, respectively. The supporting scaffolds comprise cysteine, toludine blue O as the electron mediator, and the respective enzymatic cofactor. These interfaces are now being adapted for use on electrode arrays using poly(dimethylsiloxane) microfluidic channels for fluid delivery. Cyclic voltammetry, chronoamperometry, and constant potential amperometry were used to characterize the resulting bioelectronic interfaces. The ability to fabricate devices containing multiple interfaces is expected to significantly reduce the unit cost of biosensor arrays, and facilitate new applications in drug screening.