Construction of Synthetic Human Antibody Library Based on Repertoire Sequencing
Synthetic Biology Engineering Evolution Design SEED
2021
2021 Synthetic Biology: Engineering, Evolution & Design (SEED)
Poster Session
Poster Presenters - Accepted
However, although next generation repertoire sequencing acquires hundreds of thousands of immunoglobulin repertoires from a single specimen, methods for the construction of disease-specific antibody library, its amino acid modification strategy, and functional screening has not been established to date. Here, based on repertoire information, we show a high-throughput method for the selection and synthesis of cDNA library of disease-related human antibodies and a rational designing strategy of amino acid modifications to obtain higher functional human monoclonal antibodies.
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