(320e) Construction of an Enzymatic Shuttling Compartment Based on Reverse Micellar for Bamboo Biomass Hydrolysis in Ionic Liquids | AIChE

(320e) Construction of an Enzymatic Shuttling Compartment Based on Reverse Micellar for Bamboo Biomass Hydrolysis in Ionic Liquids

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Although ionic liquids (ILs) have emerged as a promising type of solvent for lignocellulose pretreatment, the enzymatic saccharification of regenerated lignocellulose must occur separately due to the toxicity of ILs to cellulase. Therefore, it is important to develop a biocompatible IL-cellulase system in which the IL effectively activates lignocellulosic biomass, and the enzyme maintains high stability. For this purpose, a dual-phase “enzyme-shuttling compartment” was constructed in this study. Polysorbate-80 (also known as Tween 80) was found to form reverse micelles in the isooctane-IL two-liquid phase, acting as a compartment for cellulose and providing a microenvironment that protects and maintains the energetic conformation of the reactive cellulase. The activated bamboo biomass was enzymatically hydrolyzed in 20% (w/v) 1-ethyl-3-methylimidazolium dimethyl phosphate and 50 mM citrate buffer at 50 °C, and a high total reducing sugar (TRS) yield of 71.2% was obtained after 24 h. At the same time, an enzymatic activity of 91.2% was maintained after 24 hours of enzymolysis. The compartment with a diameter of 0.4 to 0.8 μm was observed by TEM. After 16 hours of enzymolysis, the enzymatic activity was maintained up to 93% in the reverse micelles system, compared to 82.3% in the ionic liquid-aqueous solution. Thus, an efficient system with the simultaneous ILs activation and enzymolysis of natural biomass was successfully developed in a one-pot procedure at low temperatures, ensuring large-scale biomass conversion into biofuels and biological products.