(320e) Construction of an Enzymatic Shuttling Compartment Based on Reverse Micellar for Bamboo Biomass Hydrolysis in Ionic Liquids
AIChE Annual Meeting
2022
2022 Annual Meeting
Innovations in Process Engineering
Breakthroughs in Sustainable Chemical Production and Process
Tuesday, November 15, 2022 - 2:30pm to 3:00pm
Although ionic liquids (ILs) have emerged as a promising type of solvent for lignocellulose pretreatment, the enzymatic saccharification of regenerated lignocellulose must occur separately due to the toxicity of ILs to cellulase. Therefore, it is important to develop a biocompatible IL-cellulase system in which the IL effectively activates lignocellulosic biomass, and the enzyme maintains high stability. For this purpose, a dual-phase âenzyme-shuttling compartmentâ was constructed in this study. Polysorbate-80 (also known as Tween 80) was found to form reverse micelles in the isooctane-IL two-liquid phase, acting as a compartment for cellulose and providing a microenvironment that protects and maintains the energetic conformation of the reactive cellulase. The activated bamboo biomass was enzymatically hydrolyzed in 20% (w/v) 1-ethyl-3-methylimidazolium dimethyl phosphate and 50 mM citrate buffer at 50 °C, and a high total reducing sugar (TRS) yield of 71.2% was obtained after 24 h. At the same time, an enzymatic activity of 91.2% was maintained after 24 hours of enzymolysis. The compartment with a diameter of 0.4 to 0.8 μm was observed by TEM. After 16 hours of enzymolysis, the enzymatic activity was maintained up to 93% in the reverse micelles system, compared to 82.3% in the ionic liquid-aqueous solution. Thus, an efficient system with the simultaneous ILs activation and enzymolysis of natural biomass was successfully developed in a one-pot procedure at low temperatures, ensuring large-scale biomass conversion into biofuels and biological products.