(164f) Amyloid Aggregation of Enzymes Under Non-Denaturing Conditions
AIChE Annual Meeting
Monday, November 14, 2022 - 3:30pm to 5:00pm
In our study presented herein, a folded enzyme, Bacillus circulans xylanase (BCX), aggregates into amyloid fibrils during incubation under a non-denaturing condition. We also found that the aggregation is concomitant with the loss of enzymatic activity and can be reduced by peptides derived from other amyloid proteins. Moreover, we found that different enzymes, including those with high conformational stability, also aggregates into amyloid fibrils under non-denaturing conditions, further supporting that amyloid aggregation of enzymes under non-denaturing conditions is more common than what has been reported. Collectively, our study enhances an understanding of amyloid aggregation of enzymes under non-denaturing conditions toward advances in industrial biochemical processes.