(116e) Grad Student Paper Award, 3rd Place: "Effect of Combustion Particle Morphology on Biological Responses in a Co-culture of Human Lung Epithelial and Macrophage-like Cells"
AIChE Annual Meeting
Monday, November 14, 2022 - 2:15pm to 2:30pm
*Methods.* This study altered the shape of lab-generated combustion particles, without affecting the chemical composition, from fiber-like to a more compact spherical shape using a water condensation-evaporation method. Using an electrostatic field-based air-liquid exposure (ALI) chamber, the two shapes were exposed to a co-culture of human airway epithelial (A549) and differentiated human monocyte (THP-1) cells at ALI conditions.
*Results.* For the same mass dose of 2 mg/cm2, both shapes were ingested by cells, and induced a pro-inflammatory response (IL-8 and TNFÎ±) and enhanced CYP1A1 gene expression compared to air controls. The more compact spherical particles (representative of atmospherically aged particles) induced more early apoptosis and release of TNFÎ± compared to the more fiber-like particles.
*Conclusions.* The result suggests a contribution of morphology to the increased toxicity of aged particles.
Effect of Combustion Particle Morphology on Biological Responses in a Co-culture of
Human Lung and Macrophage Cells, Kamaljeet Kaur, Raziye Mohammadpour, Hamidreza Ghandehari, Christopher A. Reilly, Robert Paine, Kerry E. Kelly
Atmospheric Environment 2022, 284