(150e) Detection of the Tuberculosis Volatile Organic Biomarker Methyl Nicotinate in Breath Using Electroactive Solutions (EAS)
AIChE Annual Meeting
Monday, November 8, 2021 - 1:42pm to 2:00pm
Our labâs electrochemical breath biopsy method utilizes a cobalt Engineered Electroactive Solution (EAS) to analyze samples on inexpensive, commercially available screen-printed electrodes via square wave voltammetry (SWV). The change in signal of a transition metal of known electrochemical activity is observed when the solution is exposed to an analyte of interest. These results show cobalt EAS to be both sensitive and specific to methyl nicotinate, responding to biomarker levels found in patient breath, with a limit of detention of approximately .01 mM MN. A unique peak for MN is observed with peak heights that proportionately increase with biomarker concentration. Furthermore, the use of cobalt EAS for detection of MN in mimicked patient breath samples shows that the diffusive gas to liquid transfer process adequately purified samples of potential confounders, resulting in negligible interference in detection.
 World Health Organization, âStatus of the TB epidemic,â 2019.
 Syhre, M. and Chambers, S.T., 2008. The scent of Mycobacterium tuberculosis. Tuberculosis, 88(4), pp.317-323.