(480c) Nanobiocatalytic Enzyme Stabilization for Membrane Antifouling and CO2 Conversion

Authors: 
Kim, J. - Presenter, Korea University
Enzymes are biomolecules that can catalyze a variety of chemical reactions. However, their applications are often hampered by their poor stability. Nanobiocatalysis, immobilizing enzymes using various nanomaterials, has demonstrated successes in stabilizing the enzyme activity for various enzyme applications such as biosensors, biofuel cells, enzyme-linked immunosorbent assay, membrane antifouling, and CO2 conversion. This presentation will cover nanobiocatalytic stabilization of acylase and carbonic anhydrase for their successful uses in enzyme-catalyzed quorum quenching and CO2 conversion, respectively. Especially, the stabilization of carbonic anhydrase has made an unprecedented success by maintaining 83% of initial enzyme activity even after incubation in aqueous solution under shaking at 200 rpm for two years. Stabilized carbonic anhydrase was successfully employed for the conversion of CO2 to bicarbonate, which was further used for microalgae growth and calcium carbonate production. If time permits, several other examples of nanobiocatalytic stabilization and applications will be introduced.