(459f) Construction of a Red Emission Bodipy-Based Probe for Tracing Lysosomalviscosity Changes in Culture Cells
AIChE Annual Meeting
2020
2020 Virtual AIChE Annual Meeting
Topical Conference: Sensors
Application in Bio-Sensors
Wednesday, November 18, 2020 - 9:15am to 9:30am
A red emission BODIPY-based lysosome-targeted viscosity probe (Lys-VBOD) was synthesized for monitoring lysosomal viscosity in live cells. The increase of viscosity can restrict the twisting of double bond between indole
and BODIPY fluorophore, also restrict the rotating of single bond between quinoline and BODIPY, which lead to the enhancement of fluorescence intensity in high viscosity environment. The changes of fluorescence intensity
exhibit a good linear relationship with viscosity values. Besides, Lys-VBOD is quite stable in various pH solvents with given viscosity, suggesting that Lys-VBOD is suitable for applying in a wide pH range. Moreover, Lys-VBOD
can visualize the changes of lysosomal viscosity in live cells under the stimulus of dexamethasone. BSA titration experiment demonstrated that Lys-VBOD is stable in the presence of macromolecules and proteins. Notably, the
fluorescence lifetime of Lys-VBOD in large viscosity solvents is extremely high. These results indicate that LysVBOD is an excellent tool for detecing viscosity changes in vitro and live cells.
and BODIPY fluorophore, also restrict the rotating of single bond between quinoline and BODIPY, which lead to the enhancement of fluorescence intensity in high viscosity environment. The changes of fluorescence intensity
exhibit a good linear relationship with viscosity values. Besides, Lys-VBOD is quite stable in various pH solvents with given viscosity, suggesting that Lys-VBOD is suitable for applying in a wide pH range. Moreover, Lys-VBOD
can visualize the changes of lysosomal viscosity in live cells under the stimulus of dexamethasone. BSA titration experiment demonstrated that Lys-VBOD is stable in the presence of macromolecules and proteins. Notably, the
fluorescence lifetime of Lys-VBOD in large viscosity solvents is extremely high. These results indicate that LysVBOD is an excellent tool for detecing viscosity changes in vitro and live cells.