(358c) Inverse Emulsion of Poly(acrylamide-co-itaconic acid) Nanoparticles for the Oral Delivery of Protein Therapeutics
Copolymeric nanoparticle systems containing acrylamide and itaconic acid and crosslinked with N,Nâ-methylenebisacrylamide were synthesized via inverse emulsion polymerization, a protocol adapted from Zhong et al4. Following purification with ethanol and dialysis against an ethanol:water gradient, the composition of the resulting particles was confirmed with Fourier-transform infrared spectroscopy and potentiometric titration. The surface morphology of the nanogels was evaluated using electron microscopy. Nanogel swelling was characterized with dynamic light scattering and the zeta potential was measured with electrophoretic light scattering. With dynamic light scattering, it was confirmed that the synthesized nanogels exhibit the expected swelling behavior with increasing pH values. Electrophoretic light scattering demonstrated that both formulations exhibit a negative zeta potential, with the value becoming more negative with increasing pH values. FTIR analysis of these gels showed the expected characteristic peaks and potentiometric titration suggested that particles with increasing incorporation of itaconic acid required higher volumes of hydrochloric acid to decrease the pH, indicating the presence of more carboxylic acid groups. The synthesized nanogels show promise for oral protein delivery applications. Further testing is needed, such as protein loading and release into the nanogels. In addition, strategies to enhance protein transport across the intestinal epithelium will be evaluated.
This work was supported in part by NIH grant number R01 EB022025 and the Cockrell Family Regents Chair in Engineering (UT Austin). H.F.O. was supported by the National Science Foundation Graduate Research Fellowship and the Archie W. Straiton Endowed Graduate Fellowship in Engineering #2 (UT Austin).
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