(282d) Design of Lipid-Nanoparticle Assemblies for High-Sensitive Lipid Membrane Analysis
AIChE Annual Meeting
2020 Virtual AIChE Annual Meeting
Engineering Sciences and Fundamentals
AIChE and Society of Chemical Engineers, Japan (SCEJ) Joint Session in Bio/Nano/Interfacial Phenomena II (Invited Talks)
Tuesday, November 17, 2020 - 2:00pm to 2:15pm
The aim of this study is to design SERS platforms for lipid membrane systems. Here, small (diameter: 5 nm) and large (diameter: 100 nm) diameter AuNPs were employed to induce membrane surface-enhancement Raman spectra. The SERS from AuNP5nm@lipid and AuNP100nm@lipid were compared as well as the enhancement factor (EF) values. The AuNP5nm was successfully embedded in the inner lipid membrane due to the small size and hydrophobic property. Raman spectra of the AuNP5nm@lipid showed that the signals of the fingerprint region were stronger than AuNP100nm@lipid. The Raman spectrum from AuNP5nm@lipid described the enhanced signals in the hydrophobic area which indicated that AuNP5nm was entrapped in the lipid bilayer. Finally, the differentiate Raman spectra was also compared between AuNPs and lipid and it was concluded that AuNP5nm@lipid has been exhibited enhanced peaks derived from hydrophobic region of the membrane. The cholesterol was also indicated that it was localized in the hot spot area of the AuNPs. This approach will shed lights in characterizing liquid-ordered vs liquid-disordered membrane phases, and in detecting the AuNP associated lipids in membrane systems.